Source: www.doh.gov.ph


Health Secretary Enrique T. Ona and Governor Vilma Santos Recto join hands in unveiling the official marker declaring Batangas as a Malaria-Free Province. The Department of Health made the declaration after five years of intensive internal and external evaluation and finding out that there is no trace of any local transmission. In his message, Secretary Ona commended Batangas health workers and local officials headed by Governor Vilma for their determined action and commitment to totally eradicate malaria from the province. Malaria is a life-threatening disease transmitted by mosquitoes. It affects 40% of the world’s population, putting 3.2 billion people at risk in tropical countries like the Philippines. It is one of the leading causes of deaths worldwide.

An Alleged Army Major Trying To Steal Money From Owners Of Clinical Laboratories In Bulacan

By Jeffrey V. de Guzman

Introducing himself as a dear friend of Attorney Lutero of Bureau of Health Facilities and Services Division of the Department of Health, this alleged army major tries to rip off money from clinical laboratory owners in Bulacan. This unknown man identified himself as an army major to sell tickets to the unsuspecting owners. Apparently, he was forcing them to buy three tickets that cost 6,000 pesos apiece.

The first grievance came from the owner of San Roque Drug Testing Laboratory.  According to one of her staff, she received a call from a woman saying that her boss wants to talk to the owner.  The man introduced himself as an army major and a close friend of attorney Lutero, who happens to be the chief of BHFS.  The man said that attorney Lutero want to sell three tickets to her and his representative will deliver the tickets and collect the payment.

The CHD 3 office also received information that one owner of another laboratory in Bulacan actually bought one ticket already.  And last Friday (November 26, 2010), we received another complaint from the owner of a laboratory from San Jose Del Monte City.

An investigation is underway as of this writing. On behalf of the department, we want to inform everyone that no such solicitation has been authorized and other related activities are not permitted. Through this article, please be warned and be extra careful when dealing with people who are trying to associate themselves with Atty. Lutero or any member of the department.

We are calling on everyone involved to please help us in disseminating the information to stop this fraudulent incident from happening again.

Application Form For The Renewal of License To Operate General Clinical Laboratories In Central Luzon

Below is the copy of the application form for initial and renewal of license to operate a general clinical laboratory.  This can actually be downloaded from the DOH website.  The only difference is that the list of personnel and equipment must also be submitted for the renewal of license even without changes.

This is intended for clinical laboratories under the jurisdiction of Center for Health Development 3.  Always prepare a duplicate copy of all the documents submitted.

Application For License To Operate A General Clinical Laboratory in the Philippines -

Please see the latest checklist of application documents here


By Jeffrey V. de Guzman

For those who work in a laboratory, and actually for most Filipinos from all walks of life and age groups demonstrated a trend toward increasing overweight and obesity. This is partly due to lack of regular exercise and movement of the body. This trend strengthens the urgency to promote physical activity among Filipinos.

For the majority of people, a sedentary lifestyle constitutes a major health risk. But for a very small minority, physical or sporting activity may be risk for health. Therefore, anyone who has a special health condition or illness, or suspect of having one, should consult their family physician before engaging in any physical activity or exercise.


PAMET 2010 46th Annual Convention

1:00-5:00 P.M. (THE TENT)
8:00 A.M. - 5:00 P.M. (THE TENT)

DECEMBER 1, 2010

8:00 -12:00 A.M.


8:00  12:00 AM  
Centennial A    :: Quality Control in Clinical Laboratory
Centennial B    :: Diagnostic and Prognostic markers in Sepsis
Millenium Salon    :: Overview of DSSM based on PTSI workshop`
Champagne Salon    :: Evaluation of Sperm Morphology

DECEMBER 1, 2010
1:00 – 6:00 P.M.




6:30 – 9:30 P.M.


Source: http://www.pametinc.org

Care and Maintenance of a Microscope

The Microscope                                                              

Hemoglobin Determination Methods in the Philippines

This article is intended to address the common violation of clinical laboratories with regards to haemoglobin determination.  Majority of the non-hospital based freestanding clinical laboratories specially primary categories do not even perform the test but a result is being released by multiplying a factor (0.333) to the hematocrit of the patient.  Poor patients, they are paying for the test but without knowing that it is actually not being done by the medical technologist involved.  Such thing is very unprofessional and with all the notices and warnings issued by the regulatory officers, this practice still exist.

The approved standard for quantitative blood  haemoglobin determination in the Philippines is by using the hemiglobincyanide (HiCN) or commonly known as the cyanmethemoglobin methodTo perform this method, blood is mixed with Drabkin's solution, a solution that contains ferricyanide and cyanide. The ferricyanide oxidizes the iron in the hemoglobin, thereby changing hemoglobin to methemoglobin. Methemoglobin then unites with the cyanide to form cyanmethemoglobin.  All forms of hemoglobin likely to occur in circulatory blood, with the exception of sulfhemoglobin, are determined. Cyanmethemoglobin produces a color which is measured in a colorimeter, spectrophotometer, or automated instrument.  The color is suitable for measurement in filter as well as in narrow-band spectrophotometers, because its absorption band at a wavelength of 540 nanometers is broad and relatively flat.  The color relates to the concentration of hemoglobin in the blood.  Standards prepared from either crystalline hemoglobin or washed erythrocytes and stored in a brown glass container and in sterile condition are stable for at least nine months (change 2%).

Since very few primary category clinical laboratory are equipped with hemoglobinometer  or a photometer, the regulatory officers consider manual methods which include the Haden-Hausse and Sahli-Hellige methods. In both methods, blood is mixed with dilute hydrochloric acid. This process hemolyzes the red cells, disrupting the integrity of the red cells' membrane and causing the release of hemoglobin, which, in turn, is converted to a brownish-colored solution of acid hematin. The acid hematin solution is then compared with a color standard.

This methods of determination of haemoglobin as haeminchloride (acid haematin) though not recommended because of their unreliability, are sufficient for routine examination provided they are properly done.

Below is the procedure for the Sahli-Hellige method.

Materials Required:
  • ·         Distilled water
  • ·         Sahli-helligehemoglobinometerkit containing:
o   Small bottle of dilute (approx. 0.1N) hydrochloric acid. Prepare this solution by adding 1 ml of concentrated HCl to 99 ml of distilled water. POUR ACID INTO WATER. Replenish this peri-odically–it must be of proper strength.
o   Graduated tube, with a scale on two sides. On one side is the percentage scale, and on the opposite side is the gram scale. The percentage scale reads from 0 to 170. The gram scale reads from 0 to 24.
  • ·          Pipette, marked at the 20 mm 3 level
  • ·         Stirring rod
  • ·         Color comparator, with a window in the side. On the right and left sides of this opening is the color standard for comparison. The center has an open slot to hold the graduated tube.  
  1. With a medicine dropper, place 5 drops of the 0.lN HCl in the bottom of the graduated tube. Place the tube in the color comparator.
  2. Using well-mixed venous blood or fingertip blood, fill the pipette to the 20 mm mark.
  3. Wipe blood from the outside of the pipette. Transfer blood to the Sahli tube. Note the time.
  4. Aspirate distilled water into the pipette two or three times and transfer these washings to the tube.
  5. Shake until the blood is well mixed and the tube is a uniform color.
  6. Add distilled water, drop by drop, each time mixing the solution with the stirring rod. Keep adding water and mixing until the color of the solution matches the standards on either side. Remove the stirring rod from the tube each time before com-paring. Natural light makes more accurate readings possible.
  7. Five minutes after time noted, read the result from the scale on the tube by noting the graduation mark at the lower edge of the meniscus. Read and report both scales.


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